منابع مشابه
Detection of Z DNA binding proteins in tissue culture cells.
A gel electrophoresis DNA binding assay to detect Z DNA binding proteins has been developed utilising [32P] labelled poly [d(G-C)] which was converted to the Z form by incubation in 100 microM Co(NH3)6Cl3. The parameters of the assay were established using a Z DNA antibody as a model system and then applied to extracts of Hela and BHK21 cells. Using an anti-Z DNA antibody conditions were establ...
متن کاملBinding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA.
Recognition of unusual left-handed Z-DNA by specific binding of small molecules is crucial for understanding biological functions in which this particular structure participates. Recent investigations indicate that zinc cationic porphyrin (ZnTMPyP4) is promising as a probe for recognizing Z-DNA due to its characteristic chiroptical properties upon binding with Z-DNA. However, binding mechanisms...
متن کاملZ-DNA-binding proteins can act as potent effectors of gene expression in vivo.
The role of Z-DNA-binding proteins in vivo is explored in yeast. A conformation-specific yeast one-hybrid system is made in which formation of Z-DNA is studied near a minimal promoter site where it can be stabilized by negative supercoiling in addition to protein binding. Experiments were carried out with a Z-DNA-binding protein domain from the editing enzyme, double-stranded RNA adenosine deam...
متن کاملSingle-stranded DNA-binding Proteins
The emergence of DNA as the predominant hereditary material of life has necessitated the evolution of proteins whose sole function is the maintenance and care of these vital molecules. In a typical cell, there is a wide array of proteins with specialized functions in DNA metabolism that allow the cell to maintain and replicate its genome. In order to copy or make repairs to DNA, the double heli...
متن کامل- turn - helix ; DNA - binding proteins )
The Saccharomyces cerevisiae CDC43 gene product is involved in establishing cell polarity during the cell-division cycle. When grown at restrictive temperatures, temperature-sensitive cdc43 mutants are unable to form buds and display delocalized cell-surface deposition [Adams et al., J. Cell Biol. (1990) in press]. We have isolated a cdc43-complementing plasmid from a yeast genomic-DNA library ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.23.9691